Pine St. Nature Trail is located on the east side of Millstadt. The Nature Trail has walking trails that include a paved trail and a wood chip trail. The paved trail is in a mostly shaded area. The wood chip trail incorporates shaded and non-shaded areas. This park is handicap accessible. See parking areas below.

There are two parking areas for the park. The first parking area is located on Pine Street. Take Rt. 158/Washington Street and turn onto S. Kossuth, go 1 block and turn left onto E. Madison Street and travel approximately .3 miles and turn right onto Pine Street. The park is at the end of the street. This parking area is handicap accessible starting on the paved trail. The second parking area is at the back of the Nature Trail and is located off of E. Laurel. Take the same directions above, however, on E. Madison turn right onto E. Laurel, travel approximately .2 miles and turn left into the Millstadt Sewer Plant driveway and take the turn to the left of the Sewer Plant down to an open area. You are free to park anywhere in that open area.